Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*04:01&GAD65 (NFFRMVISNPAAT) Complex Protein (Monomer, MALS verified)

• Description:

  Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*04:01&GAD65 (NFFRMVISNPAAT) Complex Protein (HL5-H82E6) is expressed from human 293 cells (HEK293) . It contains AA Ile 26 - Glu 216 (HLA-DRA1*01:01) & Gly 30 - Lys 227 (HLA-DRB1*04:01) & NFFRMVISNPAAT peptide (Accession # CAI2388006.1 (HLA-DRA1*01:01) & CAI9239961.1 (HLA-DRB1*04:01) & NFFRMVISNPAAT) .

• Specifications:

  Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*04:01&GAD65 (NFFRMVISNPAAT) Complex Protein is produced by co-expression of HLA-DRA1 and HLA-DRB1 loaded with GAD65 peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 29.6 kDa and 29.2 kDa. The protein migrates as 38-42 kDa and 33-37 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  CAI2388006.1 (HLA-DRA1*01:01) & CAI9239961.1 (HLA-DRB1*04:01) & NFFRMVISNPAAT

• Expression Region:

  Ile 26 - Glu 216 (HLA-DRA1*01:01) & Gly 30 - Lys 227 (HLA-DRB1*04:01) & NFFRMVISNPAAT peptide

• Host:

  HEK293

• Target:

  HLA-DRA1*0101 & HLA-DRB1*0401 & GAD65 (NFFRMVISNPAAT)

• Conjugation:

  Biotin-labeled

• Tag:

  C-10xHis & C-Avi

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Purity:

  90%

• Format:

  Powder

• Buffer:

  PBS, pH7.4

• Additives:

  Trehalose

• Molecular Weight:

  29.6 kDa and 29.2 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  200ug*1

• Species:

  Human