HLA Class II DRB1 Recombinant Rabbit Monoclonal Antibody [JE60-43]

• Description:

  A beta chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the alpha chain HLA-DRA, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell receptor (TCR) on HLA-DRB1-restricted CD4-positive T cells. This guides antigen-specific T-helper effector functions, both antibody-mediated immune response and macrophage activation, to ultimately eliminate the infectious agents and transformed cells. Typically presents extracellular peptide antigens of 10 to 30 amino acids that arise from proteolysis of endocytosed antigens in lysosomes. In the tumor microenvironment, presents antigenic peptides that are primarily generated in tumor-resident APCs likely via phagocytosis of apoptotic tumor cells or macropinocytosis of secreted tumor proteins. Presents peptides derived from intracellular proteins that are trapped in autolysosomes after macroautophagy, a mechanism especially relevant for T cell selection in the thymus and central immune tolerance. The selection of the immunodominant epitopes follows two processing modes: 'bind first, cut/trim later' for pathogen-derived antigenic peptides and 'cut first, bind later' for autoantigens/self-peptides. The anchor residue at position 1 of the peptide N-terminus, usually a large hydrophobic residue, is essential for high affinity interaction with MHCII molecules.

• Product Name Alternative:

  2B1F_HUMAN antibody DR1 antibody DR16 antibody DR5 antibody DR8 antibody DRB1 antibody DRw10 antibody DW2.2/DR2.2 antibody FLJ75017 antibody FLJ76359 antibody HLA class II antigen beta chain antibody HLA class II histocompatibility antigen, DR-1 beta chain antibody HLA class II histocompatibility antigen, DRB1-15 beta chain antibody HLA-DR-beta 1 antibody HLA-DR1B antibody HLA-DRB antibody HLA-DRB1 antibody human leucocyte antigen DRB1 antibody Leucocyte antigen DR beta 1 chain antibody leucocyte antigen DRB1 antibody lymphocyte antigen DRB1 antibody major histocompatibility complex, class II, DR beta 1 antibody MHC class II antigen antibody MHC class II antigen DRB1*15 antibody MHC class II antigen HLA-DR13 antibody MHC class II HLA-DR beta 1 chain antibody MHC class II HLA-DR-beta cell surface glycoprotein antibody MHC class II HLA-DRw10-beta antibody SS1 antibody

• Abbreviation:

  HLA class II histocompatibility antigen, DRB1 beta chain Human leukocyte antigen DRB1 (HLA-DRB1) HLA-DRB1

• Swiss Prot:

  P01911 Human, P01912 Human, P04229 Human, P20039 Human, Q29974 Human, Q30134 Human, Q30167 Human, Q5Y7A7 Human, Q95IE3 Human, Q9GIY3 Human

• Cellular Locus:

  Cell membrane. Endoplasmic reticulum.

• Host:

  Rabbit

• Species Reactivity:

  Human

• Immunogen:

  Synthetic peptide within Human HLA Class II DRB1 aa 151-200 / 266.

• Isotype:

  IgG

• Clone:

  JE60-43

• Conjugation:

  Non-conjugated

• Type:

  Recombinant Rabbit monoclonal Antibody

• Applications:

  WB, IHC-P, IF-Tissue

• Positive Control:

  Ramos cell lysate, Raji cell lysate, Daudi cell lysate, HDLM-2 cell lysate, A375 cell lysate, human tonsil tissue, human skin tissue, human diffuse large B-cell lymphoma tissue.

• Concentration:

  1 mg/mL

• Dilution:

  WB: 1:1,000 ;IHC-P: 1:1,000 ;IF-Tissue: 1:100

• Purity:

  Protein A affinity purified.

• Form:

  Liquid

• Buffer:

  1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Molecular Weight:

  Predicted band size: 30 kDa

• Storage Conditions:

  Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

• Recombinant Antibody:

  Yes

• CAS Number:

  9000-83-3