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Hexa-His

CAT: 0804-HY-P0294-01Size: 1 mgDry Ice: NoHazardous: No
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CAT#:0804-HY-P0294-01Size:1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Hexa-His (6X His Tag) is a commonly used affinity tag made up of six histidine residues. HEXA-HIS can bind to affinity chromatography media containing transition metal ions like nickel (Ni2+) or cobalt (Co2+), making it useful for protein purification[1][2].
CAS Number
64134-30-1
Product Name Alternative
6X His Tag
UNSPSC
12352209
Hazard Statement
H302, H315, H319
Target
Biochemical Assay Reagents
Type
Peptides
Related Pathways
Others
Field of Research
Others
Assay Protocol
https://www.medchemexpress.com/Hexa-His.html
Concentration
10mM
Purity
99.84
Solubility
H2O : 50 mg/mL (ultrasonic)
Smiles
O=C(O)[C@H](CC1=CNC=N1)NC([C@H](CC2=CNC=N2)NC([C@H](CC3=CNC=N3)NC([C@H](CC4=CNC=N4)NC([C@H](CC5=CNC=N5)NC([C@H](CC6=CNC=N6)N)=O)=O)=O)=O)=O
Molecular Formula
C36H44N18O7
Molecular Weight
840.85
Precautions
H302, H315, H319
References & Citations
[1]Watly J, et al. Insight into the coordination and the binding sites of Cu (2+) by the histidyl-6-tag using experimental and computational tools. Inorg Chem. 2014 Jul 7;53 (13) :6675-83.|[2]Ana Paula de Mattos Arêas, et al. Synthesis of cholera toxin B subunit gene: cloning and expression of a functional 6XHis-tagged protein in Escherichia coli.Protein Expr Purif.2002 Aug;25 (3) :481-7.
Shipping Conditions
Blue Ice
Storage Conditions
-80°C, 2 years; -20°C, 1 year (Powder, sealed storage, away from moisture)
Scientific Category
Peptides
Clinical Information
No Development Reported