TMB

• UNSPSC Description:

  TMB is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922[1][2].

• Target Antigen:

  Fluorescent Dye; Reactive Oxygen Species

• Type:

  Dye Reagents

• Related Pathways:

  Immunology/Inflammation;Metabolic Enzyme/Protease;NF-κB;Others

• Field of Research:

  Others

• Assay Protocol:

  https://www.medchemexpress.com/TMB.html

• Solubility:

  DMSO : 25 mg/mL (ultrasonic)

• Smiles:

  NC1=C(C)C=C(C2=CC(C)=C(N)C(C)=C2)C=C1C

• Molecular Weight:

  240.34

• References & Citations:

  [1]Bresolí-Obach R, et al. Tetramethylbenzidine: An Acoustogenic Photoacoustic Probe for Reactive Oxygen Species Detection. Sensors (Basel). 2020 Oct 21;20(20):5952. |[2]Frey A, et al. A stable and highly sensitive 3,3',5,5'-tetramethylbenzidine-based substrate reagent for enzyme-linked immunosorbent assays. J Immunol Methods. 2000 Jan 13;233(1-2):47-56.

• Shipping Conditions:

  Room Temperature

• Clinical Information:

  No Development Reported

• CAS Number:

  54827-17-7