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Methyl Green

CAT: 0804-HY-D0163-01Size: 100 mgDry Ice: NoHazardous: No
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CAT#:0804-HY-D0163-01Size:100 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Methyl Green is a non-intercalating fluorescent labeling agent that selectively binds to the major groove of DNA. Methyl Green electrostatically interacts with the major groove of DNA through positively charged groups, exhibiting key activities such as high affinity, resistance to photobleaching, and stable fluorescence emission. Methyl Green can be directly measured by microscopy and flow cytometry, with peaks at 633 and 677 nm. Methyl Green can be used for fluorescent labeling of the nuclei of embryonic tissues or cells, or DNA staining and cell activity detection in gel electrophoresis[1][2][3].
CAS Number
14855-76-6
UNSPSC
12171500
Hazard Statement
H302, H318, H351, H361, H373, H410
Target
DNA Stain
Type
Dye Reagents
Related Pathways
Cell Cycle/DNA Damage
Field of Research
Others
Assay Protocol
https://www.medchemexpress.com/methyl-green.html
Solubility
DMSO : 125 mg/mL (ultrasonic)
Smiles
C[N+](C)(CC)C1=CC=C(/C(C2=CC=C(N(C)C)C=C2)=C3C=C/C(C=C\3)=[N+](C)\C)C=C1.[Br-].[Cl-]
Molecular Formula
C27H35BrClN3
Molecular Weight
516.94
Precautions
H302, H318, H351, H361, H373, H410
References & Citations
[1]Prieto D, et al. Application of the DNA-specific stain methyl green in the fluorescent labeling of embryos. J Vis Exp. 2015 May 2; (99) :e52769.|[2]Prieto D, et, al. A fast, low cost, and highly efficient fluorescent DNA labeling method using methyl green. Histochem Cell Biol. 2014 Sep;142 (3) :335-45.|[3]Kim SK, et al. Methyl green. A DNA major-groove binding drug. FEBS Lett. 1993 Jan 2;315 (1) :61-4.
Shipping Conditions
Room Temperature
Storage Conditions
4°C (Powder, sealed storage, away from moisture and light)
Scientific Category
Dye Reagents
Clinical Information
No Development Reported

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