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Anti-GFAP Antibody

CAT: 1303-75-240Size: 100 µLDry Ice: NoHazardous: No
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CAT#:1303-75-240Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Glial fibrillary acidic protein (GFAP) is an intermediate filament with many different isoforms, and acts as a structural protein in the cytoskeleton. It is expressed in astrocytes in brain and is often used as an astrocyte marker. GFAP is belived to be involved in many CNS processes including cell communication and mitosis. GFAP has been found to be involved in Alexander disease and Wernicke's encephalopathy.
Description
Our Anti-GFAP mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N206A/8. It is KO validated, detects human, mouse, and rat GFAP, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
Short Description
Our Anti-GFAP mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N206A/8. It is KO validated, detects drosophila, guinea pig, human, mouse, polar bear, and rat GFAP, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.
Synonyms
Glial fibrillary acidic protein (GFAP)
Gene Name
GFAP
UniProt
P14136
Host
Mouse
Reactivity
Drosophila, Guinea Pig, Human, Mouse, Polar Bear, and Rat
Immunogen
Synthetic peptide amino acids 411-422 (KTVEMRDGEVIK) of human GFAP (accession number P14136)
Target
GFAP
Clonality
Monoclonal
Isotype
IgG1
Clone
N206A/8
Type
Primary Antibody
Applications
WB, IHC, ICC, ELISA
Field of Research
Glial and Schwann Cell Markers/Astrocyte Markers
Concentration
1 mg/mL
Format
Purified by Protein A chromatography
Buffer
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
Molecular Weight
50 kDa
Shipping Conditions
Shipped on ice packs
Storage Conditions
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Notes
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
IHC Dilution
1:500
WB Dilution
1:1000
Specificity
Cross-reacts with GFAP-R416W and other GFAP mutant proteins. Does not cross-react with other proteins (based on KO validation results) .
ICC Dilution
1:500
Quality Control
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
Recommended Usage
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Physical Properties
Liquid
Immunogen Species
Human
WB Brain Quality Control
1
Conjugate
Unconjugated
RRID
AB_10672299

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