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5-CFDA-AM

CAT: 0013-GTR19351107-02Size: 50 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR19351107-02Size:50 mg
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Description
5-CFDA-AM is a neutrally charged, cell-permeable esterase substrate hydrolyzed intracellularly to fluorescent carboxyfluorescein, which is retained due to its negative charge, enabling assessment of enzyme activity and membrane integrity. It is used in in vitro and in vivo research for cell viability, cytotoxicity, and flow cytometry assays.
CAS Number
124412-00-6
Molecular Weight
532.45
Storage Conditions
-20°C
Notes
For research use only.

Chemical Information

Cfda-AM

is an indirect measure of cell membrane integrity-are noninvasive and can be monitored conveniently directly in multiwell plates

CAS Number124412-00-6
PubChem CID180237
IUPAC Nameacetyloxymethyl 3',6'-diacetyloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylate
Molecular FormulaC28H20O11
Molecular Weight532.4
XLogP3.5
Topological Polar Surface Area141
Hydrogen Bond Donor Count0
Hydrogen Bond Acceptor Count11
Rotatable Bond Count9
Heavy Atom Count39
Formal Charge0
Complexity967
SMILES
CC(=O)OCOC(=O)C1=CC2=C(C=C1)C3(C4=C(C=C(C=C4)OC(=O)C)OC5=C3C=CC(=C5)OC(=O)C)OC2=O
InChI
InChI=1S/C28H20O11/c1-14(29)34-13-35-26(32)17-4-7-21-20(10-17)27(33)39-28(21)22-8-5-18(36-15(2)30)11-24(22)38-25-12-19(37-16(3)31)6-9-23(25)28/h4-12H,13H2,1-3H3
InChIKey
YDBUJZYYYBVSEF-UHFFFAOYSA-N
Chemical Structure
2D Structure
2D structure of Cfda-AM
CAS: 124412-00-6
CID: 180237
Formula: C28H20O11
MW: 532.4
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight532.4
XLogP33.5
Hydrogen Bond Donor Count0
Hydrogen Bond Acceptor Count11
Rotatable Bond Count9
Exact Mass532.10056145
Monoisotopic Mass532.10056145
Topological Polar Surface Area141 Ų
Heavy Atom Count39
Formal Charge0
Complexity967
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count1
Compound Is CanonicalizedYes

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