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Antibody Coating Buffer, 5X

CAT: 0013-GTR19348798Size: 1 LDry Ice: NoHazardous: No
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CAT#:0013-GTR19348798Size:1 L
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Antibody Coating Buffer saves reagents, enhances specific signal, and extends the shelf life of coated plates. It maximizes the adsorption of capture antibodies onto polystyrene plates and stabilizes antibody 3-D structure for optimal performance.
Concentration
5 X
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
1. Prepare standards and samples in General Serum Diluent. 2. Serum samples should generally be diluted at least 1:50 in order to minimize backgrounds caused by non-specific antibody binding. 3. To dilute the sample 1:100, add 1 part sample to 99 parts General Serum Diluent. For example, add 10 μL sample to 990 μL sample diluent for a total of 1,000 μL. 4. Highly concentrated samples may need to be diluted 1:1,000 or more. 5. Once diluted, run the assay according to the specific ELISA protocol.

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