Products for Research Use Only
RegisterLogin

Avidin/Biotin Blocking Kit Set

CAT: 0013-GTR19347165Size: 50 mLDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0013-GTR19347165Size:50 mL
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Some tissue sections contain endogenous biotin, biotin binding proteins, lectins or non-specific binding substances. Tissues may also bind avidin, biotin, peroxidase biotin, peroxidase streptavidin in avidin biotin based Immunohistochemistry. These tissues will give high background in the absence of biotinylated secondary antibodies. It may be necessary to block the tissue with avidin, followed by biotin. These reagents are added before the primary antibody.
Field of Research
Cell Biology
Notes
For research use only.
Applications Notes
IHC/ICC procedure for frozen sections, paraffin sections, cell smears. 1. Deparafinize and hydrate tissue sections through xylene or other clearing agents and graded alcohols. (For frozen sections or cell smears; use unfixed, acetone fixed or appropriate fixative for the antigen in question; for cell smears it may be necessary to permealize the cell by detergent, please refer to antibody protocol) 2. Wash 2-3 with distilled or deionized water. 3. Wash slide with Tris/saline buffer, followed by blocking with normal blocking serum, rinse with buffer. 4. Block with reagent A (Avidin) for 5-10 minute, rinse with buffer. 5. Now block with reagent B (Biotin) for 5-10 minutes, wash thoroughly with buffer. Note: If antigen retriever is required it can be applied after this stage. 6. Wash slide with PBS or Tris saline (with 0.02-0.05% nonionic detergent, Triton X100, Tween 20 or NP-40) or washing buffer (Immuno Automation buffer IBSC) 3-5X. 7. Follow instructions for IHC/ICC.
Tested Applications
ICC, IHC

Related Products

CatalogName
GTR19288783Streptavidin/Biotin Blocking Kit set
GTR19288782Streptavidin/Biotin Blocking Kit set
GTR18184091Avidin Antibody Biotin Conjugated
GTR18184089Avidin Antibody Biotin Conjugated
GTR18184088Avidin Antibody Biotin Conjugated
GTR18821512-01Avidin ELISA Kit
GTR19347164Avidin-Biotin-Peroxidase Complex for ELISA
GTR18982654-01Avidin
GTR19008654-01Avidin
GTR19347174Avidin

Alternative Products

CatalogName
E08-100Avidin-Biotin Blocking Kit
MBS635459-01Avidin (Biotin) Blocking Reagent
MBS238253-01AVIDIN/BIOTIN BLOCKING REAGENT
A4500-14Avidin/Biotin Blocking Kit, BioAssayTM
AR-6585-02Avidin/Biotin Blocking Kit Set
A4500-11Avidin/Biotin Blocking Reagent discontinued
G2790Endogenous Avidin-Biotin Blocking Kit
GWB-Q00289AVIDIN/BIOTIN BLOCKING REAGENT Reagent
ICT-AR-6585-02Avidin/Biotin Blocking Kit Set (50 ml)
IW-1301IHC-Tek Avidin/Biotin Blocking Solution, Ready To Use

Popular Products