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Beta galactosidase

CAT: 0013-GTR19347412Size: 1 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR19347412Size:1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Biotin Beta Galactosidase Conjugated
Product Name Alternative
Beta D galactosidase , Beta gal , Beta galactosidase , ECK0341 , JW0335 , actase , lacZ
Conjugation
Biotin
Applications
ELISA: 1:8,000 - 1:32,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
Concentration
1.0 mg/mL
Purity
Biotin Beta Galactosidase Conjugated was prepared from electrophoretically pure Beta Galactosidase and Biotin. Biotin Beta Galactosidase assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Beta Galactosidase (E.coli) and anti-Biotin.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing Beta Galactosidase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.
Notes
For research use only.
Applications Notes
Biotin Beta Galactosidase Conjugated has been tested by ELISA and dot blot and is assayed against 1.0 ug of Streptavidin in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 is suggested for Biotin Beta Galactosidase Conjugated.
Tested Applications
DOT, ELISA, IHC, WB
Preservative
Buffer: 0.05 M Potassium Phosphate, 0.001M Magnesium Chloride, 0.1M Beta-Mercaptoethanol; pH 7.8

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