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Blotto B Pre-Mixed

CAT: 0013-GTR19346382Size: 500 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR19346382Size:500 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Blotto B Pre-Mixed
Product Name Alternative
Blotto B Pre-Mixed, TBS, 1% non-fat dry milk, 1% BSA, 0.05% TWEEN-20, blocking reagents for western or ELISA
Conjugation
Unconjugated
Applications
ELISA: User Optimized, WB: User Optimized
Concentration
1X
Purity
This product contains highly purified immunoanalytical grade non-fat dry milk and bovine serum albumin (protease and immunoglobulin-free BSA) dissolved in a solution of Tris buffered saline.
Form
Lyophilized
Storage Conditions
Store container at room temperature prior to opening. After reconstitution, use blocking buffers immediately. Working solutions may be stored at 4° C for up to four (4) days. Solutions containing BLOTTO may be frozen. Contains TBS, 1% non-fat dry milk and 1% BSA. Adding the entire contents of the attached vial containing 25% TWEEN-20 (using a transfer pipette) to 500 ml of BLOTTO B results in a final concentration of 0.05% TWEEN-20. Mix well prior to use. The resulting buffer is a 1X working solution.
Notes
For research use only.
Applications Notes
Use Biorbyt's BLOTTO whenever procedures call for immunoanalytical grade non-fat dry milk. Alternative blocking agents such as BLOTTO A (solution of TBS, 5% non-fat dry milk and 0.05% Tween-20) and BLOTTO B (solution of TBS, 1% non-fat dry milk, 1% BSA and 0.05% Tween-20) are also available. BLOTTO A is a convenient general purpose blocking agent. BLOTTO B is recommended for blocking when phospho specific antibodies are used. BLOTTO A and BLOTTO B provide Tween-20 in a separate vial in liquid form (p/n TW0025) . Users should consider the addition of Tween-20, protease inhibitors and phosphatase inhibitors when required. Typically 50 mM NaF can be added to BLOTTO solutions to inhibit phosphatase activity. We recommend using 1X working solutions of BLOTTO A or BLOTTO B directly for blocking purposes and primary antibody dilution and incubation.
Tested Applications
WB
Preservative
None

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