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DiR Membrane Staining Kit

CAT: 0013-GTR19339080-01Size: 50 assayDry Ice: NoHazardous: No
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CAT#:0013-GTR19339080-01Size:50 assay
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Highly deep red fluorescent membrane probe for labeling live and fixed cells
CAS Number
100068-60-8
Field of Research
Cell Biology
Form
Liquid
Molecular Weight
1013.4
Storage Conditions
Shipped at 4°C. Store at -20°C and protect from light for 12 months.
Notes
For research use only.
Applications Notes
1. For adherent cells staining (1) Adherent cells were cultured on a sterile cover slide. (2) Remove the cover glass from the medium, absorb excess liquid but keep the surface moist. (3) Add 100 μL of Working Solution to one corner of the cover glass and gently shake to evenly cover all cells. (4) Cells were incubated at 37 °C for 2-20 mins. The reaction time can be optimized to obtain uniform labeling effect. (5) Discard Working Solution, wash the glass with PBS for 2 to 3 times. 2. For suspension cells staining (1) Adding an appropriate volume of Working Solution to re-suspension cells, the density of the cells is 1x 106 /mL. (2) The cells were incubated at 37°C for 2-20 min. The reaction time can be optimized to obtain uniform labeling effect. (3) After incubation, centrifuge at 1000-1500 rpm for 5 mins. Discard the supernatant and slowly add the growth medium again to resuspend the cells. (4) Repeat step (3) more than twice.
Tested Applications
IF

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