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Dna Ligase T4

CAT: 0013-GTR19338758Size: 100 UnitsDry Ice: NoHazardous: No
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CAT#:0013-GTR19338758Size:100 Units
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Dna Ligase T4
Product Name Alternative
DNA Ligase T4 Molecular Biology Grade
Conjugation
Unconjugated
Field of Research
Molecular Biology
Concentration
6 weiss units/μL
Bioactivity
6.0 units/μL
Form
Liquid
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
One unit will catalyze the exchange of one nanomole 32-P from pyrophosphate into a Norit-absorbable compound in 20 minutes at 37° C (Weiss, B., et al., JBC 243, 4543 (1968) . 50 mM Tris-HCl, 10 mM MgCl2, 10 mM DTT, 1 mM ATP, pH 7.5 @ 25°C.
Preservative
Buffer: See application note.

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