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ELISA Wash Buffer, 10X

CAT: 0013-GTR19337880Size: 1 LDry Ice: NoHazardous: No
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CAT#:0013-GTR19337880Size:1 L
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
ELISA Wash Buffer effectively rinses ELISA plates between reagent addition steps to remove signal-altering debris yet preserve assay components to reduce background and increase signal. Suitable for antibody-sandwich ELISAs and antigen-down ELISAs.
Concentration
10 X
Storage Conditions
Room temp
Notes
For research use only.
Applications Notes
When using HRP Conjugate Stock Stabilizer, 5X as a Conjugate Diluent:, 1. Add 1 part HRP Conjugate Stock Stabilizer, 5X to 4 parts deionized water and mix for 5-15 minutes. 2. Add the antibody-HRP or protein-HRP conjugate directly into the conjugate diluent to a working 1X dilution and mix for 5-15 minutes. 3. Pipette diluted conjugate onto the plate, or store protected from light at 2-8°C for use at a later date. 1X working conjugate dilutions can be stored at 2-8°C, protected from light, providing a useful shelf-life of 6-12 months. When using HRP Conjugate Stock Stabilizer, 5X as a Concentrated Conjugate Stock Storage Buffer:, 1. To purified HRP conjugate, add an equal volume of the 5X HRP Conjugate Stock Stabilizer. 2. Filter sterilize into a sterile amber or brown-glass container. 3. Store at 2-8°C. Concentrated HRP conjugate stocks should be stable for up to 18 months.

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