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Fluorescein

CAT: 0013-GTR19335193-02Size: 500 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR19335193-02Size:500 mg
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Description
Fluorescein (Solvent Yellow 94) is a synthetic, fluorescent phthalic dye widely employed as a tracer. It is extensively used in both in vitro assays and in vivo research for applications such as vascular permeability studies, angiography, and cellular staining due to its bright green fluorescence upon excitation.
CAS Number
2321-07-5
Product Name Alternative
Ig kappa chain V-II region RPMI, Yellow fluorescein, Resorcinol phthalein, Solvent Yellow 94, Uranine
Purity
88.59% (May vary between batches)
Solubility
10% DMSO+40% PEG300+5% Tween-80+45% Saline:2.5 mg/mL (7.52 mM) ; DMSO:60 mg/mL (180.55 mM)
Molecular Weight
332.31
Storage Conditions
-20°C
Notes
For research use only.

Chemical Information

Fluorescein

Fluorescein (lactone form) is a xanthene dye that is highly fluorescent, detectable even when present in minute quantities. Used forensically to detect traces of blood, in analytical chemistry as an indicator in silver nitrate titrations and in microscopy. It has a role as a radioopaque medium and a fluorescent dye. It is a polyphenol, a gamma-lactone, a xanthene dye, an oxaspiro compound, an organic heteropentacyclic compound and a member of 2-benzofurans. It is functionally related to a fluorescein (acid form).

CAS Number2321-07-5
PubChem CID16850
IUPAC Name3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one
Molecular FormulaC20H12O5
Molecular Weight332.3
XLogP3.4
Topological Polar Surface Area76
Hydrogen Bond Donor Count2
Hydrogen Bond Acceptor Count5
Rotatable Bond Count0
Heavy Atom Count25
Formal Charge0
Complexity522
SMILES
C1=CC=C2C(=C1)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O
InChI
InChI=1S/C20H12O5/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20/h1-10,21-22H
InChIKey
GNBHRKFJIUUOQI-UHFFFAOYSA-N
Chemical Structure
2D Structure
2D structure of Fluorescein
CAS: 2321-07-5
CID: 16850
Formula: C20H12O5
MW: 332.3
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight332.3
XLogP33.4
Hydrogen Bond Donor Count2
Hydrogen Bond Acceptor Count5
Rotatable Bond Count0
Exact Mass332.06847348
Monoisotopic Mass332.06847348
Topological Polar Surface Area76 Ų
Heavy Atom Count25
Formal Charge0
Complexity522
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count1
Compound Is CanonicalizedYes

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