Anti-GIT1 Antibody

• Description:

  Mouse Monoclonal Antibody specific to GIT1

• Product Name Alternative:

  Anti-GIT1 Mouse Monoclonal Antibody

• Gene Name:

  Git1

• NCBI Gene ID:

  Git1

• UniProt:

  Q9Z272.1

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Fusion protein corresponding to aa 375-770 (C-terminus) of rat GIT1 (accession no.Q9Z272).

• Target Antigen:

  ARF GTPase-activating protein GIT1

• Target:

  GIT1

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Type:

  Antibody

• Applications:

  WB, ICC/IF, IP

• Field of Research:

  Neuroscience

• Purification Method:

  Purified by Protein G affinity chromatography

• Concentration:

  1.0 mg/mL

• Dilution:

  Dilute in PBS or medium that is identical to that used in the assay system.

• Format:

  Purified

• Form:

  Liquid

• Buffer:

  Phosphate Buffered Saline

• Additionnal Information:

  Immunoblotting: use at 1ug/mL. Predicted molecular weight is ~90kDa.
  
  Positive control: rat brain lysate.
  
  These are recommended concentrations.
  
  Endusers should determine optimal concentrations for their applications.

• Storage Conditions:

  This antibody is stable for at least one (1) year at -20°C.

• Specificity:

  This antibody recognizes human, mouse and rat GIT1. It does not cross-react with GIT2.

• Formulation:

  PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.

• Buffer pH:

  pH 7.4

• Target Background:

  G-protein coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT1 and GIT2) are highly conserved ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains for small GTPases, kinases, the Rho family GEF PIX, and the focal adhesion protein paxillin. GIT1 is localized to focal adhesions, cyto- plasmic complexes and membrane protrusions, and it regulates cell protrusion formation and cell migration.

• CAS Number:

  9007-83-4