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Anti-GIT1 Antibody

CAT: 0014-56537-1Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0014-56537-1Size:100 µg
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Description
Mouse Monoclonal Antibody specific to GIT1
CAS Number
9007-83-4
Product Name Alternative
Anti-GIT1 Mouse Monoclonal Antibody
Gene Name
Git1
NCBI Gene ID
Git1
UniProt
Q9Z272.1
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Fusion protein corresponding to aa 375-770 (C-terminus) of rat GIT1 (accession no.Q9Z272).
Target Antigen
ARF GTPase-activating protein GIT1
Target
GIT1
Clonality
Monoclonal
Isotype
IgG1
Type
Antibody
Applications
WB, ICC/IF, IP
Field of Research
Neuroscience
Purification Method
Purified by Protein G affinity chromatography
Concentration
1.0 mg/mL
Dilution
Dilute in PBS or medium that is identical to that used in the assay system.
Format
Purified
Form
Liquid
Buffer
Phosphate Buffered Saline
Additionnal Information
Immunoblotting: use at 1ug/mL. Predicted molecular weight is ~90kDa.

Positive control: rat brain lysate.

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.
Storage Conditions
This antibody is stable for at least one (1) year at -20°C.
Specificity
This antibody recognizes human, mouse and rat GIT1. It does not cross-react with GIT2.
Formulation
PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.
Buffer pH
pH 7.4
Target Background
G-protein coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT1 and GIT2) are highly conserved ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains for small GTPases, kinases, the Rho family GEF PIX, and the focal adhesion protein paxillin. GIT1 is localized to focal adhesions, cyto- plasmic complexes and membrane protrusions, and it regulates cell protrusion formation and cell migration.

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