Products for Research Use Only

Methyl Green

CAT: 0013-GTR19315069Size: 100 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR19315069Size:100 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This methyl green counterstain solution is designed for nuclear counterstain after completion of Immunohistochemistry staining or for routine histology.
Storage Conditions
Room Temperature
Notes
For research use only.
Applications Notes
A. Immunohistochemistry: 1. After chromogen staining, wash slide 2-3X with de/di water, 2X with buffer and 2X with de/di. water 2. Apply Methyl Green counterstain for 3-5 minutes or longer (if deep green color is required, slide may be incubated at 60oC for 2-5 minutes) . 3. DO NOT rinse with water. Go directly into 95 % alcohol for few dips and 100% alcohol 5-7X. 4. Clear with Xylene or clearing agent. 5. The slides can be mounted with Organic mounting mediums like Organo mount (limonene) or equivalent. B. Histology: A. Follow the procedure as outlined for hitology 1. Deparafinize formalin-fixed paraffin-embedded (FFPE) tissue sections. 2. At the counterstain step, apply Methyl Green counterstain for 3-5 minutes or longer (if deep green color is required, slide may be incubated at 60oC for 2-5 minutes) . 3. DO NOT rinse with water. Go directly into 95 % alcohol for few dips and 100% alcohol 5-7X. 4. Clear with Xylene or clearing agent. 5. The slides can be mounted with Organic mounting mediums like Organo mount or equivalent.
Tested Applications
IHC