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Methyl Green

CAT: 0246-AR-6526-01Size: 15 mLDry Ice: NoHazardous: No
CAT#:0246-AR-6526-01Size:15 mL
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PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This methyl green counterstain solution is designed for nuclear counterstain after completion of Immunohistochemistry staining or for routine histology.
Applications
IHC
Assay Principle
A. Immunohistochemistry: 1. After chromogen staining, wash slide 2-3X with de/di water, 2X with buffer and 2X with de/di. water 2. Apply Methyl Green counterstain for 3-5 minutes or longer (if deep green color is required, slide may be incubated at 60ºC for 2-5 minutes). 3. DO NOT rinse with water. Go directly into 95 % alcohol for few dips and 100% alcohol 5-7X. 4. Clear with Xylene or clearing agent. 5. The slides can be mounted with Organic mounting mediums like Organo mount (limonene) or equivalent. B. Histology: A. Follow the procedure as outlined for hitology 1. Deparafinize formalin-fixed paraffin-embedded (FFPE) tissue sections. 2. At the counterstain step, apply Methyl Green counterstain for 3-5 minutes or longer (if deep green color is required, slide may be incubated at 60ºC for 2-5 minutes). 3. DO NOT rinse with water. Go directly into 95 % alcohol for few dips and 100% alcohol 5-7X. 4. Clear with Xylene or clearing agent. 5. The slides can be mounted with Organic mounting mediums like Organo mount (ImmunoBioscience cat. # AR-6504) or equivalent.
Shipping Conditions
Ambient Temperature
Storage Conditions
Room Temperature

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