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Rabbit Mouse IgG2b (subclass specific) Antibody

CAT: 0013-GTR17700869Size: 10 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR17700869Size:10 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
IgG fraction of polyclonal rabbit antiserum to Mouse IgG2b, subclass specific. As unlabelled primary or secondary reagent for indirect detection of IgG2b at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user's own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Typical working dilutions in histochemistry are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5.000.
Reactivity
Mouse
Clonality
Polyclonal
Conjugation
Unconjugated
Source
Pools of purified homogenous IgG2b isolated from pooled mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Form
Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) . No preservative added, as it may interfere with the antibody activity.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Host or Source
Rabbit

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