Rat IgG1 (Immunoglobulin G1) ELISA Kit

• Abbreviation:

  IgG1

• Cross Reactivity:

  This kit recognizes IgG1 in samples.No significant cross-reactivity or interference between IgG1 and analogues was observed.

• Target:

  IGHG1

• Type:

  Competitive-ELISA

• Applications:

  ELISA

• Field of Research:

  Immunology; Cell Biology

• Assay Principle:

  This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with IgG1. During the reaction, IgG1 in samples or Standard competes with a fixed amount of IgG1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to IgG1. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of IgG1 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  2 h 30 min

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  0.31-20 μg/mL

• Precision:

  Both intra-CV and inter-CV are < 10%.

• Recovery:

  80%-120%

• Sensitivity:

  0.19 μg/mL

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  2-8°C,12 months

• Detection Instrument:

  Microplate reader

• Species:

  Rat

• Recommended Usage:

  This ELISA kit applies to the in vitro quantitative determination of Rat IgG1 concentrations in serum, plasma and other biological fluids.