Rat MTL (Motilin) ELISA Kit

Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Dry Ice Shipment: No

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CAT:

763-E-EL-R0639-01

Size:

96 Tests

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Rat MTL (Motilin) ELISA Kit

Abbreviation: MTL
Synonyms: MLN
UniProt: A8IRIN/A
Species Reactivity: Rat
Type: Competitive-ELISA
Applications: ELISA
Field of Research: Signal transduction
Detection Method: Colormetric
Assay Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat MTL. During the reaction, Rat MTL in the sample or standard competes with a fixed amount of Rat MTL on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat MTL. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat MTL in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Performance Time: 2 h 30 min
Detection Range: 93.75-6000 pg/mL
Sensitivity: 56.25 pg/mL
Shipping Conditions: Ice bag
Storage Conditions: 2-8°C/-20°C, 12 months

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