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DIG-11-dUTP

CAT: 0013-GTR19237490Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR19237490Size:25 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Digoxigenin-11-dUTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Digoxigenin-labeled DNA/cDNA probes are subsequently detected using Digoxigenin-antibodies conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP) or a fluorescent dye. Optimal substrate properties and thus labeling efficiency is ensured by a 11-atom linker attached to the C5 position of uridine. Recommended Digoxigenin-11-dUTP/dTTP ratio for PCR and Nick Translation: 35% Digoxigenin-11-dUTP/ 65% dTTP Please note: The optimal final concentration of Digoxigenin-11-dUTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Digoxigenin-11-dUTP/dTTP ratio is recommended.
Product Name Alternative
Digoxigenin-X- (5-aminoallyl) -2'-deoxyuridine-5'-triphosphate, Triethylammonium salt, alkali-stable
Concentration
1.0 mM - 1.1 mM
Purity
≥ 95 % (HPLC)
Form
Filtered solution (30 kDa) in 10 mM Tris-HCl, Color: colorless to slightly yellow
Smiles
C1[C@@H]2[C@] (CC[C@@H]1OCC (=O) NCCCCCC (=O) NC/C=C/c1c (=O) [nH]c (=O) n ([C@@H]3O[C@H] (COP (=O) (OP (=O) (OP (=O) (O) O) O) O) [C@@H] (O) C3) c1) ([C@@H]1[C@@H] (CC2) [C@@]2 ([C@] ([C@@H] (C1) O) ([C@H] (CC2) C1=CC (=O) OC1) C) O) C
Molecular Formula
C43H65N4O21P3 (free acid)
Molecular Weight
1066.91 g/mol (free acid)
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
Notes
For research use only.
Applications Notes
Incorporation into DNA/cDNA by - PCR with Taq polymerase [1,2] & in-house data - Nick Translation with DNAse I/ DNA Polymerase I [3] and in-house data - Primer Extension with Klenow exo- [4] - 3’-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) [5] - Reverse Transcription with MMLV Reverse Transcriptase [6] Incorporation into RNA by - 3’-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) [7], Spectroscopic Properties: λmax 290 nm, ε 8.8 L mmol-1 cm-1 (Tris-HCl pH 7.5)

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