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1,4-Chrysenequinone

CAT: 0013-GTR19217000-01Size: 100 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR19217000-01Size:100 mg
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Description
1,4-Chrysenequinone is an activator of aryl hydrocarbon receptor (AhR) . (In Vitro) :Mouse hepatoma (H1L1.1c2) cells (1.5 x 105 cells/well) are cultured in 96-well culture plates, and samples (1,4-Chrysenequinone) dissolved in dimethyl sulfoxide are added to the medium to achieve a final solvent concentration of 1%. After the plates are incubated at 37°C in 5% CO2 for 24 h, the cell viability is confirmed under a microscope. Subsequently, the medium is removed, and the cells are lysed. Adding luciferin as the substrate, the luciferase activity is determined under a luminometer and reported as relative light units. The concentrations producing luciferase activity equal to 25% of the maximal response to TCDD are calculated and also referred to as the ECTCDD25. The ratios of the ECTCDD25 of B[a]P to the ECTCDD25 of each of the compounds are calculated and referred to as IEFs. 1,4-Chrysenequinone shows AhR ligand activity, with ECTCDD25s (concentration equivalent with 25% of TCDD max) of 9.7 nM and 1.9 μM in yeast and mouse hepatoma cell systems, respectively.
CAS Number
100900-16-1
Product Name Alternative
Chrysene-1,4-dione
Purity
>98% (HPLC)
Solubility
In Vitro: DMSO : 3.33 mg/mL (12.89 mM)
Smiles
O=C1C=CC (=O) c2c1ccc1c2ccc2ccccc12
Molecular Formula
C18H10O2
Molecular Weight
258.276
Storage Conditions
Storage temperature: -20°C. Stability: ≥ 2 years
Notes
For research use only.

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