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ONPG

CAT: 0013-GTR19216728Size: 1 gDry Ice: NoHazardous: No
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CAT#:0013-GTR19216728Size:1 g
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Description
ONPG is a colorimetric and spectrophotometric substrate used to detect β-galactosidase activity. (In Vitro) :To investigate the kinetic parameters of recombinant enzyme, the Michaelis-Menten constants (Km), turnover numbers (kcat), and catalytic efficiencies (kcat/Km) of Gal308 for ONPG and lactose are determined. The kcat and Km values are 464.7±7.8/s and 2.7±0.3 mM for ONPG, and 264.2±2.1/s and 7.1±0.8 mM for lactose, respectively. The kcat/Km value of the enzyme for ONPG (172.1 /s/mM) is 4.6-fold higher than that for lactose (37.2 /s/mM), which clearly demonstrated that the catalytic efficiency of Gal308 for ONPG is much higher than that for lactose. Gal308 displays high hydrolysis ability for ONPG (100%) and moderate activity for its natural substrate lactose (25.7%) . However, the hydrolysis ability of the enzyme towards all other chromogenic nitrophenyl analogues is very weak, indicating that Gal308 is a β-galactosidase with narrow substrate specificity.
CAS Number
369-07-3
Product Name Alternative
2-Nitrophenyl β-D-galactopyranoside
Purity
>98% (HPLC)
Solubility
In Vitro: H2O : 8.33 mg/mL (27.65 mM)
Smiles
OC[C@H]1O[C@@H] (Oc2ccccc2[N+] ([O-]) =O) [C@H] (O) [C@@H] (O) [C@H]1O
Molecular Formula
C12H15NO8
Molecular Weight
301.251
Storage Conditions
Storage temperature: -20°C. Stability: ≥ 2 years
Notes
For research use only.

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