5-Ethynyl-dUTP (5-EdUTP)

CAT: 0013-GTR19207932Size: 10 ulDry Ice: NoHazardous: No

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CAT#:0013-GTR19207932Size:10 ul

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Description

5-Ethynyl-dUTP (5-EdUTP)

Product Name Alternative

5-Ethynyl-2'-deoxyuridine 5'-triphosphate, Sodium salt

Concentration

100 mM-110 mM

Purity

95% (HPLC)

Form

Solution in water; Color: colorless to slightly yellow; pH: 7.5 0.5

Smiles

C1 (=O) [nH]c (=O) n (cc1C#C) [C@H]1C[C@H] (O) [C@H] (O1) COP (=O) (O) OP (=O) (O) OP (=O) (O) O

Molecular Formula

C11H15N2O14P3

Molecular Weight

Theoretical MW: 492.16 g/mol (free acid) ; Detected MW: 491.97 g/mol (free acid)

Storage Conditions

Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible

Notes

For research use only.

Applications Notes

Incorporation into DNA by PCR with family B polymerases (Pwo, Deep Ventexo-or KOD XL) . Incorporation into DNA by Primer Extension with family A polymerase Taqand family B polymerases (Pwo, Deep Vent exo-or KOD XL) . The resulting ethynyl-functionalized DNA can subsequently be processed via Cu (I) -catalyzed click chemistry that offers the choice: to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks; to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging; to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins. Spectroscopic Propertie: max 288 nm, 12.0 L mmol-1 cm-1 (Tris-HCl, pH 7.5) .

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Alternative Products

Catalog	Name
CLK-T07-L	5-Ethynyl-dUTP (5-EdUTP)
JBS-CLK-T07-S	5-Ethynyl-dUTP (5-EdUTP) (5 μl)
JBS-CLK-T07-XL	5-Ethynyl-dUTP (5-EdUTP) (50 μL)
JBS-CLK-T07-L	5-Ethynyl-dUTP (5-EdUTP) (5 x 5 μl)
M0045	5-EdUTP (500 µl)
MBS433557-01	Rhodamine-5-dUTP (Rho-5-dUTP)
B8092-.01	5-Iodo-dUTP
B8082-.05	5-Br-dUTP
B8162-.025	Cyanine 5-dUTP
B8115-.05	5-Propargylamino-dUTP

5-Ethynyl-dUTP (5-EdUTP)

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