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Biotin-11-dATP

CAT: 0013-GTR19207544Size: 10 ulDry Ice: NoHazardous: No
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CAT#:0013-GTR19207544Size:10 ul
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Description
Biotin-11-dATP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dATP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal sub-strate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the 7-Deaza position of adenine.
Product Name Alternative
γ-[N- (Biotin-6-amino-hexanoyl) ]-7-propargylamino-2'-deoxy-7-deaza-adenosine-5'-triphosphate, Triethylammonium salt
Concentration
1.0 mM-1.1 mM
Purity
≥ 95% (HPLC)
Form
Filtered solution (30 kDa) in 10 mM Tris-HCl; Color: colorless to slightly yellow; pH: 7.5 ± 0.5
Smiles
N1C (=O) N[C@@H]2[C@H]1[C@H] (CCCCC (=O) NCCCCCC (=O) NCC#Cc1cn (c3ncnc (N) c13) [C@@H]1O[C@H] (COP (=O) (O) OP (=O) (O) OP (=O) (O) O) [C@@H] (O) C1) SC2
Molecular Formula
C30H45N8O15P3S
Molecular Weight
Theoretical MW: 882.71 g/mol (free acid) ; Detected MW: 882.19 g/mol (free acid)
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
Notes
For research use only.
Applications Notes
Incorporation into DNA/cDNA by: PCR with Taqpolymerase & in-house data; Nick Translation with DNAse I/DNA Polymerase I; Primer Extension with Klenow exo-; Reverse Transcription with MMLV Reverse Transcriptase. Spectroscopic Propertie: λmax 280 nm, ε 12.7 L mmol-1 cm-1 (Tris-HCl, pH 7.5) .

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Alternative Products

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D3165-0211-dATP (Biotin) (Biotin-11-dATP)
NU-1175-BIOX-SBiotin-11-dATP
NU-1175-BIOX-HCBiotin-11-dATP - high concentration
JBS-NU-1175-BIOX-LBiotin-11-dATP, L pack
JBS-NU-1175-BIOX-HCBiotin-11-dATP – high concentration
JBS-NU-1175-BIOX-SBiotin-11-dATP, S pack
NU-835-BIO14-lBiotin-14-dATP
NU-835-BIO-SBiotin-7-dATP
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NU-256LEtheno-dATP (ε-dATP)

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