5-Hydroxymethyl-dCTP

CAT: 0013-GTR19207152Size: 100 µLDry Ice: NoHazardous: No

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CAT#:0013-GTR19207152Size:100 µL

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Description

5-Hydroxymethylated DNA probes can be used as sequencing control or for pull-down of 5-hmC binding proteins from cellular lysate.

CAS Number

7226-77-9

Product Name Alternative

5-hmdCTP, 5-Hydroxymethyl-2'-deoxycytidine-5'-triphosphate, Sodium salt

Concentration

100 mM-110 mM

Purity

≥ 95% (HPLC), < 0.5% dCTP (HPLC)

Form

Solution in water; Color: colorless to slightly yellow; pH: 7.5 ± 0.5

Smiles

Nc1c (cn (c (=O) n1) [C@@H]1O[C@H] (COP (=O) (OP (=O) (OP (=O) (O) O) O) O) [C@@H] (O) C1) CO

Molecular Formula

C10H18N3O14P3

Molecular Weight

Theoretical MW: 497.18 g/mol (free acid) ; Detected MW: 497.00 g/mol (free acid)

Storage Conditions

Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible

Notes

For research use only.

Applications Notes

Incorporation into DNA by-PCR with Taqpolymerasein-house data. Spectroscopic Propertie: λmax 275 nm, ε 7.8 L mmol-1 cm-1 (Tris-HCl, pH 7.5) .

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