Rat Anti-Mouse CD19-SPRD

The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 °C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 °C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 °C. Do not freeze!
The allophycocyanin (APC), Spectral Red (SPRD), R-phycoerythrin-Cyanine 7 (R-PE-CY 7) and Rphycoerythrin- Cyanine 5.5 (R-PE-CY 5.5) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 °C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 °C.
The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 °C.
The Alexa Fluor 488 (AF488) conjugate is supplied as 0.1mg in 0.2mL of PBS/NaN3. Store at 2-8 °C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 °C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
CD19 is a monomeric transmembrane glycoprotein expressed at relatively constant levels throughout B cell development from early pro-B/pre-B cells (i.e. B220^+/CD43^+/HSA^+) through fully differentiated B cell stages. ^1-7 Terminally differentiated plasma cells do not express CD19. ^1 In humans, the CD19 molecule on the surface of mature B cells associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to provide signal transduction and promote cellular activation. ^3, 4 All splenic and peritoneal IgM^+ cells of both B-1 and B-2 lineages are CD19^+, with B-1 cells expressing higher levels of CD19 than B-2 cells in these sites.1, 7 Recent studies with CD19-deficient mice have suggested that this molecule may not be required for normal generation and maturation of B cells in the bone marrow. ^7 Monoclonal antibody 6D5 recognizes the same epitope as the published clone, 1D3. ^1, 8
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