FIX&PERM Sample Kit (CE)
Lysis of red cells might be impossible for various reasons. In such instances it is recommended to isolate mononuclear cells (MNC) via density gradient centrifugation prior to staining.
Results will be correct and reproducible as long as the procedures used respect the technical recommendations and obey good laboratory practice.
The FIX&PERM® solutions are provided in a concentration that will allow to fix and permeabilize human hematopoietic cells. It is therefore strongly recommended to stick to the working protocol in terms of concentration and volume regarding cells and antibody. The properties of FIX&PERM® have been determined using EDTA anti-coagulated peripheral blood.
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