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SHH Antibody

CAT: 0013-GTR17699385Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR17699385Size:100 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Goat polyclonal antibody to SHH
Product Name Alternative
SHH, sonic hedgehog homolog (Drosophila), HGNC:10848, HHG1, HLP3, HPE3, SMMCI , sonic hedgehog
Reactivity
Human, Mouse
Target
SHH
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
DSEALHPLGMAVK
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
P-ELISA: 1: 32000
Molecular Weight
49.6
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
WB: Approx 48kDa band observed in Human Liver lysates (calculated MW of 49.6kDa according to NP_000184.1 Recommended concentration: 1-3μg/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat Liver for this particular . Notes: Immunofluorescence: Strong expression of the protein seen in the membranes of HeLa, U251 and NIH3T3 cells. Recommended concentration: 10μg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concen
Prediction Reactivity
Canine, Rat
Tested Applications
ELISA, FC, IF, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
6469

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