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Azide-PEG3-FLAG

CAT: 0013-GTR19153132Size: 5 x 0.5 umolDry Ice: NoHazardous: No
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CAT#:0013-GTR19153132Size:5 x 0.5 umol
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Azide-PEG 3-FLAG enables the FLAG-tag (DYKDDDDK) attachment to any terminal Alkyne-or Cyclooctyne (e.g. DBCO) -functionalized molecule via Cu (I) -catalyzed terminal Alkyne-Azide Click Chemistry (CuAAC) or Cu (I) -free strain-promoted Alkyne-Azide Click Chemistry (SPAAC), respectively. The resulting FLAG-tagged molecules can subsequently be de-tected by an anti-FLAG antibody that is either immobilized onto a matrix (for purification) or coupled with a fluorescent dye or reporter enzyme for direct or indirect detection, respectively. Molecule solu-bility and efficient FLAG-tag detection is ensured by the integrated PEG-linker. Azide-FLAG (without PEG-linker) has been successfully used for the detection of Azide-tagged glycoproteins in cell lysates (final concentration: 100 M) . This concentrations may serve as a starting point for individual assay set-up.
Product Name Alternative
Azide-PEG3-FLAGtag (DYKDDDDK)
Field of Research
Cell Biology
Purity
95% (HPLC)
Form
Solid; Color: white to off-white
Solubility
Water, PBS (up to 30 mM tested)
Molecular Formula
C59H88N16O27S
Molecular Weight
Theoretical MW: 1485.49 g/mol; Detected MW: 1484.57 g/mol
Storage Conditions
Store at -20 C
Notes
For research use only.

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