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5-Ethynyl-uridine (5-EU)

CAT: 0013-GTR19153070Size: 10 mgDry Ice: NoHazardous: No
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CAT#:0013-GTR19153070Size:10 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Ethynyl-labeled uridine (5-EU) can be used as a replacement for BrU (5-Bromo-uridine) to measure de novo RNA synthesis in proliferating cells. 5-EU is cell permeable and incorporates into nascent RNA instead of its natural analog uridine. The resulting ethynyl-functionalized RNA can subsequently be detected via Cu (I) -catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging. Long term storage at < -20C. If stored as recommended, Biorbyt guarantees optimal performance of this product for 12 months after date of delivery.
CAS Number
69075-42-9
Product Name Alternative
5-Ethynyl-uridine
Purity
99% (HPLC)
Form
Solid; Color: white to off-white
Solubility
Up to 200 mM in 1x PBS or water by heating the obtained, suspension for 1 minute by 70 C, less soluble in DMSO
Smiles
N1 (cc (c (=O) [nH]c1=O) C#C) [C@H]1[C@@H] ([C@@H] ([C@H] (O1) CO) O) O
Molecular Formula
C11H12N2O6
Molecular Weight
Theoretical MW: 268.22 g/mol; Detected MW: 268.07 g/mol
Storage Conditions
Store at -20 C
Notes
For research use only.
Applications Notes
RNA synthesis monitoring. Spectroscopic Propertie: max 286 nm, 12.0 L mmol-1 cm-1 (Tris-HCl, pH 7.5) .

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Alternative Products

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