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Biotin-16-dUTP

CAT: 0013-GTR19152579Size: 5 x 200 ulDry Ice: NoHazardous: No
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CAT#:0013-GTR19152579Size:5 x 200 ul
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Biotin-16-dUTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dTTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detec-tion of the Biotin moiety is ensured by a 16-atom linker attached to the C5 position of uridine. Recommended Biotin-16-dUTP/dTTP ratio for PCR and Nick Translation: 50% Biotin-16-dUTP/50% dTTP.
Product Name Alternative
Biotin-16- (5-aminoallyl) -dUTP, Biotinyl-ε-aminocaproyl-γ-aminobutyryl-5- (3-aminoallyl) -2'-deoxyuridine-5'-triphosphate, Triethylammonium salt
Concentration
1.0 mM-1.1 mM
Purity
≥ 98% (HPLC)
Form
Filtered solution (30 kDa) in water; Color: colorless to slightly yellow; pH: 7.5 ± 0.5
Smiles
C1[C@H]2[C@@H] ([C@@H] (S1) CCCCC (=O) NCCCCCC (=O) NCCCC (=O) NC/C=C/c1c (=O) [nH]c (=O) n ([C@H]3C[C@@H] ([C@H] (O3) COP (=O) (OP (=O) (OP (=O) (O) O) O) O) O) c1) NC (=O) N2
Molecular Formula
C32H52N7O18P3S
Molecular Weight
Theoretical MW: 947.78 g/mol (free acid) ; Detected MW: 947.23 g/mol (free acid)
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Notes
For research use only.
Applications Notes
Incorporation into DNA/cDNA by: PCR with Taqpolymerase & in-house data; Nick Translation with DNAse I/DNA Polymerase I & in-house data; Primer Extension with Klenow exo-; 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) ; Reverse Transcription with MMLV Reverse Transcriptase. Incorporation into RNA by 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) . Spectroscopic Propertie: λmax 240 nm, ε 10.7 L mmol-1 cm-1 (Tris-HCl, pH 7.5) .

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