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Biotin-16-dCTP

CAT: 0013-GTR19152554Size: 5 x 200 ulDry Ice: NoHazardous: No
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CAT#:0013-GTR19152554Size:5 x 200 ul
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Biotin-16-dCTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detec-tion of the Biotin moiety is ensured by a 16-atom linker attached to the C5 position of cytidine. Recommended Biotin-16-dCTP/dCTP ratio for PCR: 50% Biotin-16-dCTP/50% dCTP.
Product Name Alternative
Biotin-16-Propargylamino-dCTP, γ-[N- (Biotin-6-amino-hexanoyl-6-aminobutanoyl) ]-5- (3-propargylamino) -2'-deoxycytidine-5'-triphosphate, Triethylammonium salt
Concentration
1.0 mM-1.1 mM
Purity
≥ 95% (HPLC)
Form
Liquid
Molecular Formula
C32H51N8O17P3S (free acid)
Molecular Weight
Theoretical MW: 944.78 g/mol (free acid) ; Detected MW: 944.23 g/mol (free acid)
Storage Conditions
Store at -20 °C
Notes
For research use only.
Applications Notes
Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase I; Primer Extension with Klenow exo-; 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) ; Reverse Transcription with MMLV Reverse Transcriptase. Spectroscopic Propertie: λmax 294 nm, ε 9.3 L mmol-1 cm-1 (Tris-HCl, pH 7.5) .
Preservative
Filtered solution (30 kDa) in 10 mM Tris-HCl. Color: colorless to slightly yellow. pH: 7.5 ± 0.5

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