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JBS True Blue

CAT: 0013-GTR19152108Size: 300 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR19152108Size:300 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Crystallization screening with high concentrations of precipitant and salt may lead to the formation of salt crystals. It is quite difficult to make a distinction between these false positives and true protein crystals. Staining of crystals with appropriate dyes is a very straightfor-ward method to differentiate between macromolecular crystals and salt crystals. Protein and salt crystals differ substantially in their solvent content. Small crystal dyes, like Biorbyt True Blue, are able to permeate the solvent channels of a protein, thus coloring the protein blue. In contrast, salt crystals are tightly packed and do not possess large solvent channels. They will therefore remain colourless.
CAS Number
61-73-4
Product Name Alternative
Methylene Blue
Concentration
4 mM
Smiles
[Cl-].s1c2-c (nc3c1cc (N (C) C) cc3) ccc (=[N+] (C) C) c2
Molecular Formula
C16H18ClN3S * H 2O
Molecular Weight
Theoretical MW: 319.85 g/mol (anhydrous)
Storage Conditions
Store at ambient temperature
Notes
For research use only.
Applications Notes
Biorbyt True Blue is a crystal dye used to stain macromolecular crystals, i.e. protein, peptide and nucleic acid crystals in order to differentiate them from small molecules and salt crystals.

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