Rat PDE5A (Phosphodiesterase 5A, cGMP Specific) ELISA Kit

Rat PDE5A (Phosphodiesterase 5A, cGMP Specific) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PDE5A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PDE5A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PDE5A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PDE5A in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: CGB-PDE; cGMP-binding cGMP-specific phosphodiesterase; cGMP-specific 3',5'-cyclic phosphodiesterase
• UniProt: O54735
• Field of Research: Enzyme & Kinase.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 10 ng/mL
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Detection Range: 0.16-10 ng/mL
• Sensitivity: 0.074 ng/mL