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Recombinant Human FGFb Protein

CAT: 0013-GTR19126129-01Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR19126129-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recombinant Human Fibroblast Growth Factor 2/Fibroblast Growth Factor Basic is produced by our E.coli expression system and the target gene encoding Gly132-Ser288 is expressed.
Product Name Alternative
Fibroblast Growth Factor 2; FGF-2; Basic Fibroblast Growth Factor; bFGF; Heparin-Binding Growth Factor 2; HBGF-2; FGF2; FGFB
UniProt
P09038
Expression Region
Gly132-Ser288
Tag
Tag free
Field of Research
Cardiovascular Research
Endotoxin
< 0.01 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (Regularly tested)
Bioactivity
BLI: Loaded Human FGFb (157AA) (Cat#orb3002481) on APS Biosensor, can bind Human GPC3-Fc (Cat#orb2993899) with an affinity constant of 0.7 nM as determined in BLI assay. (Regularly tested) . Cell Based Assay: Measured in a cell proliferation assay using BALB/c 3T3 cells.The ED50 for this effect is 0.42 ng/ml (Regularly tested) .
Form
Lyophilized
Molecular Weight
Predicted: 17.4 KDa. Observed: 16 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM Tris, 150mM NaCl, 3% Trehalose, 4% Mannitol, pH 7.5

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