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Recombinant Human IL-7 Protein

CAT: 0013-GTR19126072-01Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR19126072-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recombinant Human Interleukin-7 is produced by our E.coli expression system and the target gene encoding Asp26-His177 is expressed.
Product Name Alternative
Interleukin-7; IL-7; IL7
UniProt
P13232
Expression Region
Asp26-His177
Tag
Tag free
Field of Research
Immunology & Inflammation
Endotoxin
< 0.01 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (Regularly tested)
Bioactivity
BLI: Loaded Human IL-7RA-His (Cat#orb2993835) on HIS1K Biosensor, can bind Human IL-7 (Cat#orb3002429) with an affinity constant of 15.04 nM as determined in BLI assay. Loaded Human IL-7RA-Fc-His (Cat#orb2994563) on HIS1K Biosensor, can bind Human IL-7 (Cat#orb3002429) with an affinity constant of 18.59 nM as determined in BLI assay. Loaded Mouse IL-7RA-Fc (Cat#orb2994406) on Protein A Biosensor, can bind Human IL-7 (Cat#orb3002429) with an affinity constant of 29.2 nM as determined in BLI assay. . Cell Based Assay: Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL) . The ED50 for this effect is 0.02-0.08 ng/ml.
Form
Lyophilized
Molecular Weight
Predicted: 17.5 KDa. Observed: 18 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM PB, 300mM NaCl, pH 8.0.

UniProtKB · P13232

Interleukin-7

IL7_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P13232
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
IL7
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
IL-7
EC number
—
Processing
Precursor
Secondary accessions
A0N0L3, Q5FBY5, Q5FBY9
Protein keywords

Technical term

3D-structureReference proteome

Coding sequence diversity

Alternative splicing

Molecular function

CytokineGrowth factor

Disease

Disease variant

PTM

Disulfide bondGlycoprotein

Cellular component

Secreted

Domain

Signal

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