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Recombinant Human IL-23 Protein

CAT: 0013-GTR19124911-01Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR19124911-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recombinant Human Interleukin-23 is produced by our Mammalian expression system and the target gene encoding Arg20-Pro189&Ile23-Ser328 is expressed with a 6His tag at the C-terminus.
Product Name Alternative
SGRF; IL-23p19; CLMF p40; IL-12 subunit p40; NKSF2
UniProt
Q9NPF7, P29460
Expression Region
Arg20-Pro189&Ile23-Ser328
Conjugation
Unconjugated
Tag
C-6His
Origin
Human
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 90% as determined by SEC-HPLC. (QC verified)
Bioactivity
ELISA: Immobilized Human IL-23R-Fc (Cat#orb2994313) at 10μg/ml (100 μl/well) can bind Human IL-23-His (Cat#orb2993911) .The ED50 of Human IL-23-His (Cat#orb2993911) is 0.248 ug/ml.. BLI: Loaded Ustekinumab on Pro-A Biosensor, can bind Recombinant Human IL-23 (C-6His) (Cat#orb2993911) with an affinity constant of 40.7 pM as determined in BLI assay. (Regularly tested) Loaded Human IL-23R-Fc (Cat#orb2994313) on Protein A Biosensor, can bind Human IL-23-His (Cat#orb2993911) with an affinity constant of 0.98 nM as determined in BLI assay. Loaded Anti-Human IL23-P19 mAb-Fc (Cat#orb2993116) on Protein A Biosensor, can bind Human IL-23-His (Cat#orb2993911) with an affinity constant of 2.2 pM as determined in BLI assay. . Cell Based Assay: Measured by its ability to induce STAT reporter activity in 293F human embryonic kidney cells. The ED50 for this effect is 70-210 ng/ml. (QC verified)
Form
Lyophilized
Molecular Weight
Predicted: 19.7&34.8 KDa. Observed: 19-21&40-60 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM PB, 200mM Trehalose, 4% Mannitol, 50mM NaCl, 0.02% Tween80, pH 7.5.

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