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Recombinant Human APRIL Protein

CAT: 0013-GTR19124664-01Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR19124664-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recombinant Human A proliferation-inducing Ligand is produced by our Mammalian expression system and the target gene encoding Lys112-Leu250 is expressed with a His, Flag tag at the N-terminus.
Product Name Alternative
Tumor necrosis factor ligand superfamily member 13; A proliferation-inducing ligand; APRIL; TNF- and APOL-related leukocyte expressed ligand 2; TALL-2; TNF-related death ligand 1; TRDL-1; CD256; TNFSF13
UniProt
O75888
Expression Region
Lys112-Leu250
Conjugation
Unconjugated
Tag
N-Flag-His
Origin
Human
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Human APRIL -Flag-His (Cat#orb2994084) at 1μg/ml (100 μl/well) can bind Cynomolgus BCMA-Fc (Cat#orb2994273) .The ED50 of Cynomolgus BCMA-Fc (Cat#orb2994273) is 4.23 ng/ml.. BLI: Loaded Cynomolgus BCMA-Fc (Cat#orb2994273) on Protein A Biosensor, can bind Human APRIL-His (Cat#orb2994084) with an affinity constant of 0.005 nM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 50 KDa. Observed: 60 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM PB, 1M NaCl, pH 8.3.

UniProtKB · O75888

Tumor necrosis factor ligand superfamily member 13

TNF13_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O75888
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
TNFSF13
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A8MYD5, B4DVT2, Q541E1, Q5U0G8, Q96HV6, Q9P1M8, Q9P1M9
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoprotein

Molecular function

Cytokine

Biological process

Immunity

Cellular component

Secreted

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