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Recombinant Human Eukaryotic elongation factor 2 kinase (EEF2K)

CAT: 0013-GTR19123705-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR19123705-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This Recombinant Human Eukaryotic elongation factor 2 kinase (EEF2K) spans the amino acid sequence from region 2-725aa. Purity: Greater than 85% as determined by SDS-PAGE.
Product Name Alternative
Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase
UniProt
O00418
Expression Region
2-725aa
Tag
C-terminal 6xHis-tagged
Source
E.coli
Origin
Homo sapiens (Human)
Field of Research
Signal Transduction
Purity
Greater than 85% as determined by SDS-PAGE.
Form
Liquid or Lyophilized powder
Molecular Weight
88.9 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes
For research use only.
Applications Notes
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Preservative
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
AA Sequence
ADEDLIFRLEGVDGGQSPRAGHDGDSDGDSDDEEGYFICPITDDPSSNQNVNSKVNKYYSNLTKSERYSSSGSPANSFHFKEAWKHAIQKAKHMPDPWAEFHLEDIATERATRHRYNAVTGEWLDDEVLIKMASQPFGRGAMRECFRTKKLSNFLHAQQWKGASNYVAKRYIEPVDRDVYFEDVRLQMEAKLWGEEYNRHKPPKQVDIMQMCIIELKDRPGKPLFHLEHYIEGKYIKYNSNSGFVRDDNIRLTPQAFSHFTFERSGHQLIVVDIQGVGDLYTDPQIHTETGTDFGDGNLGVRGMALFFYSHACNRICESMGLAPFDLSPRERDAVNQNTKLLQSAKTILRGTEEKCGSPQVRTLSGSRPPLLRPLSENSGDENMSDVTFDSLPSSPSSATPHSQKLDHLHWPVFSDLDNMASRDHDHLDNHRESENSGDSGYPSEKRGELDDPEPREHGHSYSNRKYESDEDSLGSSGRVCVEKWNLLNSSRLHLPRASAVALEVQRLNALDLEKKIGKSILGKVHLAMVRYHEGGRFCEKGEEWDQESAVFHLEHAANLGELEAIVGLGLMYSQLPHHILADVSLKETEENKTKGFDYLLKAAEAGDRQSMILVARAFDSGQNLSPDRCQDWLEALHWYNTALEMTDCDEGGEYDGMQDEPRYMMLAREAEMLFTGGYGLEKDPQRSGDLYTQAAEAAMEAMKGRLANQYYQKAEEAWAQMEE

UniProtKB · O00418

Eukaryotic elongation factor 2 kinase

EF2K_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O00418
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
EEF2K
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
eEF-2 kinase, eEF-2K
EC number
2.7.11.20
Processing
—
Secondary accessions
Q8N588
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationPhosphoprotein

Ligand

ATP-bindingCalciumNucleotide-binding

Molecular function

Calmodulin-bindingKinaseSerine/threonine-protein kinaseTransferase

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E2213-55EEF2k, Phosphorylated (Ser366) (eEF2k (Eucharyotic Elongation Factor-2 kinase, eEF-2 kinase)
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MBS6294459-01Eef2k, NT (Eef2k, Eukaryotic elongation factor 2 kinase, Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase) (FITC)
MBS6294458-01Eef2k, NT (Eef2k, Eukaryotic elongation factor 2 kinase, Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase) (Biotin)
MBS6294457-01Eef2k, NT (Eef2k, Eukaryotic elongation factor 2 kinase, Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase) (APC)

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