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Recombinant Human Monocyte differentiation antigen CD14 (CD14), partial (Active)

CAT: 0013-GTR19122297-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR19122297-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This Recombinant Human Monocyte differentiation antigen CD14 (CD14), partial (Active) spans the amino acid sequence from region 20-344aa. Purity: Greater than 95% as determined by SDS-PAGE.
Product Name Alternative
Monocyte differentiation antigen CD14; My23 antigen; Myeloid cell-specific leucine-rich glycoprotein; CD14
UniProt
P08571
Expression Region
20-344aa
Tag
C-terminal 10xHis-tagged
Source
Mammalian cell
Origin
Homo sapiens (Human)
Field of Research
Immunology & Inflammation; Stem Cell & Developmental Biology
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Purity
Greater than 95% as determined by SDS-PAGE.
Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Human CD14 at 2 μg/mL on an Nickel Coated plate can bind Anti-CD14 recombinant antibody. The EC50 is 2.148-2.447 ng/mL.
Form
Lyophilized powder
Molecular Weight
37.8 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes
For research use only.
Applications Notes
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Preservative
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
AA Sequence
TTPEPCELDDEDFRCVCNFSEPQPDWSEAFQCVSAVEVEIHAGGLNLEPFLKRVDADADPRQYADTVKALRVRRLTVGAAQVPAQLLVGALRVLAYSRLKELTLEDLKITGTMPPLPLEATGLALSSLRLRNVSWATGRSWLAELQQWLKPGLKVLSIAQAHSPAFSCEQVRAFPALTSLDLSDNPGLGERGLMAALCPHKFPAIQNLALRNTGMETPTGVCAALAAAGVQPHSLDLSHNSLRATVNPSAPRCMWSSALNSLNLSFAGLEQVPKGLPAKLRVLDLSCNRLNRAPQPDELPEVDNLTLDGNPFLVPGTALPHEGSM

UniProtKB · P08571

Monocyte differentiation antigen CD14

CD14_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P08571
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CD14
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q53XT5, Q96FR6, Q96L99, Q9UNS3
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Cellular component

Cell membraneGolgi apparatusMembraneSecreted

PTM

Disulfide bondGlycoproteinGPI-anchorLipoprotein

Biological process

ImmunityInflammatory responseInnate immunity

Domain

Leucine-rich repeatRepeatSignal

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