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Recombinant Human PD-1 Protein

CAT: 0013-GTR19125310-01Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR19125310-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recombinant Human Programmed Cell Death Protein 1 is produced by our Mammalian expression system and the target gene encoding Pro21-Gln167 is expressed with a mouse IgG1 Fc tag at the C-terminus.
Product Name Alternative
Programmed cell death protein 1; hPD-1; PDCD1; CD279
UniProt
Q15116
Expression Region
Pro21-Gln167
Conjugation
Unconjugated
Tag
C-mFc
Origin
Human
Field of Research
Cancer Biology
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 90% as determined by SEC-HPLC. (QC verified)
Bioactivity
ELISA: Immobilized Anti-Human PD-1 mAb (Cat#GMP-A085) at 2μg/ml (100 μl/well) can bind Human PD-1-mFc (Cat#orb2994924) . The ED50 of Human PD-1-mFc (Cat#orb2994924) is 12.53 ng/ml.. BLI: Loaded Anti-Human PD-1 mAb-Fc (Cat#GMP-A085) on AHC Biosensor, can bind Human PD-1-His (Cat#orb2994924) with an affinity constant of -3 nM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 42 KDa. Observed: 60 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.