Products for Research Use Only

SOX2 Goat Polyclonal Antibody

CAT: 0013-GTR17698663Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR17698663Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
SOX2 Goat Polyclonal Antibody recognizes the N-terminus of human SOX2. Validated for ELISA (1:32,000), WB (0.1-0.3 μg/ml), IHC (6-8 μg/ml), and flow cytometry (10 μg/ml) . Reactivity includes human, with predicted reactivity for bovine, canine, and rat. For research use only.
Product Name Alternative
SOX2; SRY (sex determining region Y) -box 2; ANOP3; MGC2413 ; SRY-related HMG-box gene 2; sex-determining region Y-box 2; transcription factor SOX2
UniProt
P48431
Reactivity
Human
Immunogen
Peptide with the sequence YNMMETELKPPGPQQ-C, derived from the N-terminus of the protein sequence corresponding to NP_003097.1.
Target
SOX2
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology; Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
P-ELISA: 1: 32000, WB: 0.1-0.3μg/ml, IHC: 6-8μg/ml, FC: 10ug/ml
Molecular Weight
34.3 kDa
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
WB: Approx 40kDa band observed in HEK293 and MCF7 cell lysates and in nuclear lysates of cell line MCF7 (calculated MW of 34.3kDa according to) NP_003097.1) . This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3μg/m. IHC: Paraffin embedded Human Tonsil. Recommended concentration: 6-8μg/ml.. Notes: Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.
Prediction Reactivity
Bovine, Canine, Rat
Tested Applications
ELISA, FC, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.