Casp 3/7 FAM-DEVD-FMK 100 Tests

• Description:

  Non-cytotoxic assay arrests further apoptotic activity via caspase inhibition., Cell permeablity permits direct visualization of cytosolic apoptotic events., Apoptotic cell population does not diminish over time., Add reagent directly to cells. No special buffer or media needed. No preparation of cell lysates required. Simple wash procedure., Works in diverse cell lines: human, rodent, Drosophila., Can be performed in conjunction with Annexin staining, TUNEL, antibody staining, or with other APO LOGIX reagents on the same population of cells., Permits high through-put screening. Protocol can be adapted for ex vivo as Well as in situ experiments., Yields both quantitative and qualitative results. Gives strong signal with little background noise.

• Target:

  Caspase-3/7

• Label:

  ICT

• Cell Type:

  Jurkat

• Type:

  Cell Viability

• Detection Method:

  Flow Cytometry, Fluorescence Plate Reader, Fluorescence Microscopy

• Assay Protocol:

  https://www.celltechnology.com/wp-content/uploads/2020/12/FAMFMKProtocol.pdf

• Components:

  Lyophilized FAM Labeled Peptide (FAM-DEVD-FMK), 4 vials (Part 8002), 10X Apoptosis Wash Buffer, 60 mL (Part # 3026), Fixative, 6 mL (Part # 3027), Propidium Iodide, 1 mL (Part# 4013)

• Shipping Conditions:

  Ships overnight (domestic), International Priority Shipping

• Storage Temperature:

  2-8°C

• Target Description:

  APO LOGIX Carboxyfluoroscein Caspase Detection Kits label active caspases in living cells undergoing apoptosis. Cell Technology’s probes utilize carboxyfluorescein(FAM)-labeled peptide fluoromethyl ketone (FMK) caspase inhibitors (FAM-peptide-FMK). These FAM-peptide-FMK compounds are both cell permeable and non-cytotoxic during the course of the assay and thus allow the detection of active caspases in living cell systems.