Antigen-Down Assay Diluent

• Description:

  Reduces the effects of the sample matrix and variation among samples. Pre-dilution of the samples is not necessary., Reduces background noise caused by nonspecific interactions between the sample matrix proteins and the plate surface.

• Label:

  ICT

• Type:

  ELISA & Assay Reagents

• Assay Protocol:

  Once your plate has been coated with an antibody or antigen, aspirate or dump out the coating solution. Then, simply pipette 300-400uL per Well of block buffer and let it incubate (Cell Technology recommends a minimum incubation of 3 hours).

• Concentration:

  1X

• Buffer:

  7.4 at 1X

• pH:

  7.4 at 1X

• Shipping Conditions:

  Ships overnight (domestic), International Priority Shipping

• Storage Temperature:

  2-8°C

• Shelf Life:

  Expires two years from date of manufacture

• Target Description:

  ICT’s Antigen-Down Assay Diluent is specifically formulated for testing plasma and serum samples in antigen-down ELISA formats. Proprietary additives, including EDTA, have been incorporated into this diluent that eliminate the risk of plasma sample clots in the ELISA plate Well while also minimizing nonspecific binding interactions associated with these complex matrices.