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ABTS 1-Component HRP MicroWell Substrate (SUBA)

CAT: 0436-6278Size: 100 mLDry Ice: NoHazardous: No
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CAT#:0436-6278Size:100 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
ABTS 1-Component HRP MicroWell Substrate is a ready-to-use solution containing 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) in a mildly acidic buffer. This substrate is ideal for use in ELISAs where the detection level is in the µg-ng/mL range and horseradish peroxidase (HRP) is the conjugated detection enzyme. ABTS 1-Component HRP MicroWell Substrate is suitable for ELISAs where the test samples contain high concentrations of the target molecule or when it is advantageous to utilize lower sample dilution factors.
Label
ICT
Type
ELISA & Assay Reagents
Assay Protocol
1. Run ELISA according to the specific protocol through the conjugate incubation step., 2. Wash the Wells three or four times with 1X ELISA Wash Buffer (catalog #652) to remove any residual, AP-conjugate., 3. Bring SUBP to room temperature; protect from light., 4. Pipette 100 µL SUBP into each Well of the plate., 5. Incubate SUBP 10-60 minutes. Monitor the color intensity., 6. Read the plate between 405-420 nm and analyze. Alternatively, stop the reaction by adding 100 µL/Well STOPP (catalog #6284) and read the samples between 405-420 nm within 1 hour.
Concentration
1X
Additionnal Information
ELISA & Assay Reagents ; Substrates
Shipping Conditions
Ships overnight (domestic), International Priority Shipping
Storage Temperature
2-8°C
Notes
20% discount
Shelf Life
3 years from date of manufacture
Target Description
ABTS 1-Component HRP MicroWell Substrate is a ready-to-use solution containing 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) in a mildly acidic buffer. This substrate is ideal for use in all ELISAs where the target detection level is in the µg-ng/mL range and horseradish peroxidase (HRP) is the conjugated detection enzyme. ABTS 1-Component HRP MicroWell Substrate is suitable for ELISAs where the test samples contain high concentrations of the target molecule or when it is advantageous to utilize lower sample dilution factors., , ABTS substrate is oxidized by the peroxidase enzyme to yield a soluble blue-green reaction product. In endpoint assays, the reaction can be stopped by adding equal volumes of 1% sodium dodecyl sulfate (SDS)., , Bulk volumes are available upon request.

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