RegisterLogin

DAPI Nuclear Stain

CAT: 0436-6244Size: 10 mgDry Ice: NoHazardous: No
CAT#:0436-6244Size:10 mg
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Description
DAPI is a blue fluorescent DNA stain that forms complexes with natural double-stranded DNA. Use it to detect DNA and visualize nuclear morphology
Label
ICT
Type
Cell Viabiity
Detection Method
Flow cytometry, Fluorescence microscope
Wavelength
358 nm / 461 nm
Assay Protocol
1. Dilute Antigen Coating Buffer, 5X 1:5 using deionized water and mix for 15 minutes. As Antigen Coating Buffer is a 5X concentrate, crystalline precipitates may form in the bottle, especially when refrigerated. If this happens, gently warm the buffer until all crystals are dissolved. Do not let it boil., 2. Dilute your antigen into the coating buffer. Optimal coating concentration varies significantly from 0.2 µg/mL to 10 µg/mL., 3. Let the solution stir 10 – 15 minutes and pipette onto the plate. Optimal coating volume generally ranges from 50 – 200 µL per Well. ICT recommends coating antibodies onto Immulon II HB plates., 4. Once added to the plate, incubate the coating solution from 8 – 24 hours at room temperature protected from light. Minimize evaporation by individually covering each plate with a plate sealing cover, wrapping a stack in plastic wrap, or placing plates in a humidified storage box and covering. , 5. After incubation, dump or aspirate the coating solution out of the Wells., 6. Wash the plate twice with ICT’s ELISA Wash Buffer., 7. Aspirate and pipette one of ICT’s Blocking Buffers onto the plate at a higher volume than the coating solution (300 – 400 µL per Well)., 8. Once added to the plate, incubate the block buffer from 8 – 24 hours at room temperature protected from light. Minimize evaporation by individually covering each plate with a plate sealing cover, wrapping a stack in plastic wrap, or placing plates in a humidified storage box and covering., 9. Aspirate the block buffer., 10. The assay can be run at this point, or the plate can be dried and packaged for later use., 11. Dry the plate by letting it sit in the fume hood covered with foil overnight, or dry in a drying chamber under vacuum from 3 – 6 hours at room temperature. When dry, seal the plate in an air-tight foil pouch with a desiccant and store at 2-8°C protected from light.
Shipping Conditions
Ships overnight (domestic), International Priority Shipping
Storage Temperature
-20°C
Notes
13% discount
Cellular Imaging & Detection
Cellular Imaging
Target Description
DAPI is a blue fluorescent DNA stain that forms complexes with natural double-stranded DNA. Use it to detect DNA and visualize nuclear morphology.

Related Products

CatalogName
AR-6501-01Fluoroshield™ with DAPI
AR-6501-02Fluoroshield™ with DAPI
AR-6505-01Fluoroshield with DAPI & DABCO
AR-6506-01Fluoroshield with DAPI & Propylgallate
AR-6506-02Fluoroshield with DAPI & Propylgallate
AR-6505-02Fluoroshield with DAPI & DABCO
AR-6513-02Fluoroshield with DAPI & PPD
AR-6513-01Fluoroshield with DAPI & PPD
638Propidium Iodide Stain
AR-6510-02Glycerol mounting medium with DAPI and DABCO

Alternative Products

CatalogName
6244DAPI Nuclear Stain
OM643160DAPI stain Solution
RA20036-01DAPI Nuclear Dye
C0068DAPI Fixed Cell Stain (10 ml)
#23009-TDrop-n-Stain EverBriteTM Mounting Medium with DAPI
2996-1mgDAPI, blue fluorescent nucleic acid stain, 1 mg
2996-10mgDAPI, blue fluorescent nucleic acid stain, 10 mg
MBS258073-01DAPI blue fluorescent nuclear DNA dye
G1035-100MLNuclear Fast Red Stain
RA20035-01DAPI Nuclear Staining Solution (Ready-to-use)

Popular Products