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BlokHen® Blocking Reagent

CAT: 0588-BH-1001Size: 100 mLDry Ice: NoHazardous: No
CAT#:0588-BH-1001Size:100 mL
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PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Our proprietary blocking reagent, BlokHen® is a fish serum-based immunoreagent designed for use with chicken antibodies. It can be used in WB, IHC, ICC, IP, and ELISA.
Description
Our proprietary blocking reagent, BlokHen® is a fish serum-based immunoreagent designed for use with chicken antibodies. It already contains concentrated buffer and non-ionic detergent (tween-20), allowing simple dilution with water. BlokHen® can be used in Western Blots, Immunohistochemistry or Immunocytochemistry, Immunoprecipitation, or ELISA applications.
Other Statements
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Immunogen
n/a
Immunogen Type
n/a
Target
n/a
Clonality
n/a
Applications
IHC, ICC, WB
Assay Type
Blocking Reagent
Assay Protocol
Usage Notes: BlokHen IITM is meant to be diluted with phosphate buffered isotonic saline (PBS) . The extent of dilution will vary from one application to another but should be in the range of 1:5 to 1:20. Western Blots: After transferring proteins from your gel onto nitrocellulose, incubate the membrane with diluted BlokHen IITM for 1 hour at room temperature with gentle agitation. The blots should then be washed for 15 minutes with PBS prior to addition of primary antibody. It is not necessary to dilute your chicken antibodies or secondary anti-chicken IgY reagents with BlokHen IITM. Instead, dilute these reagents in PBS with 0.1% (w/v) of a non-ionic detergent, such as triton X-100 or TWEEN-20. Immunocytochemistry: In a manner similar to that described above for western blots, pre-block your paraformaldehyde-fixed cultured cells or tissue sections with diluted BlokHen IITM for 1 hour at room temperature. Then, wash off the BlokHen IITM with PBS prior to addition of chicken antibodies (diluted in PBS with 0.1% non-ionic detergent) .
Format
Liquid
Buffer
Contains 0.02% (w/v) sodium azide as preservative.
Precautions
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Shipping Conditions
Ambient
Storage Conditions
Store at room (ambient) temperature. Do not put in the refrigerator or freezer, otherwise crystals will form which can cause background problems. At room temperature, BlokHen should have a shelf life of at least 12 months.
Shelf Life
12 months from receipt of product
Quality Control
BlokHen was analyzed by enzyme-linked immunosorbent assays using nitrocellulose membranes.
Brid
AB_2313509
Physical Properties
Liquid

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