SHARPIN Polyclonal Antibody

• Background:

  Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is recruited to the TNF-R1 signaling complex (TNF-RSC following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with OTULIN, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis.

• Description:

  This is a SHARPIN Polyclonal Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  SHARPIN, SIPL1, sharpin

• Gene ID:

  81858

• Swiss Prot:

  Q9H0F6

• Reactivity:

  Human, Rat

• Immunogen:

  Recombinant fusion protein of human SHARPIN

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Polyclonal Antibody

• Applications:

  WB

• Purification Method:

  Affinity purification

• Assay Type:

  Antibody

• Concentration:

  1mg/mL

• Dilution:

  WB 1:500-1:2000

• Buffer:

  PBS with 0.01% thiomersal,50% glycerol, pH7.3.

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  34kDa/56kDa

• Observed Molecular Weight:

  56kDa

• Host or Source:

  Rabbit

• Isotype:

  IgG